STUDY OF PHYSICAL AND ANTIMICROBIAL PROPERTIES OF BIOGENIC SILVER NANOPARTICLES SYNTHESIZED VIA GREEN ROUTE

Abstract

Health is the primary concern of human beings. Apart from the problem of living and food, health issues have always been a concern for humanity. Various types of hereditary and epidemic diseases are the greatest adversaries of human health. The main cause of their generation and spread is different types of germs. Bacteria are one of the main causes of human health problems, and various medications have been used to treat it. Different treatments have been adopted to deal with the bacteria problems. The use of nanomaterial’s for therapeutic purposes, as in other areas of life, have been blessings and encouraging in recent times and is a hope for future.  The current research work was focused to synthesize silver nanoparticles from lemon leaves extract and explore their antibacterial activity on different types of bacteria. Leaves extract worked as a reducing agent to form Ag^o atoms, which further clustered to form silver nanoparticles. These silver nanoparticles were characterized by using X-Ray Diffraction (XRD) analysis, UV-Visible Spectroscopy and Scanning Electron Microscopy (SEM). The UV-Visible absorption spectra of two different prepared silver nanoparticles samples were obtained and it was observed that these samples have prominent absorption response at energetic end of visible light from 380 nm to 410 nm. The crystalline nature of synthesized silver nanoparticles was confirmed by X-Ray diffraction analysis and showed the diffraction peaks at 31.86^o, 37.74^o, 43.3^o, 64.14^o, and 77.08^o, corresponding to silver element. The antibacterial activity was tested against different pathogenic bacteria and it was observed that the average inhibition zone diameter was 10 – 13 mm which shows the positive control. The green synthesis method is very economical, eco-friendly, and simple to implement for synthesis of silver nanoparticles